anti myhc antibody  (R&D Systems)

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Comparative analysis of growth performance and pectoral muscle development of postnatal broiler chickens (n = 6). A The sampling time of AA chickens at day 1 after hatching (D1), D7, D14, D21, D28, D42, D56 and TY chickens at D1, D7, D14, D21, D28, D42, D56, D77, D105. B The changes in body weight of AA and TY chickens. C The changes in pectoral muscle weight of AA and TY chickens. D Immunofluorescence staining of myofibers with MYH1A and MYH7B of AA and TY chickens. Scale bars: 20 μm. E Statistical analysis of immunofluorescence staining density values for pectoral muscle fibers in AA and TY chickens. Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Sampling, Immunofluorescence, Staining

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Intestinal microbiota transplantation (IMT) affects the growth and pectoral muscle development of chickens. A IMT experimental design. B The variation of body weight of AA and TY chickens during D1 to D28 (n = 6). C Pectoral muscle weight of AA and TY chickens at D21 and D28 (n = 12). d -E Morphology of the myofibers stained by hematoxylin and eosin and the statistics of muscle fiber diameter (MDia) and muscle fiber density (MDen) at D21( D ) and D28 ( E ). F-G The mRNA expression of MYH1A and MYH7B in the pectoral muscle at D21 ( F ) and D28 ( G ) (n = 6). Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Transplantation Assay, Staining, Expressing

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: TLCA regulates mitochondrial biogenesis and altered myofiber type composition through the p38 MAPK/PGC-1α signaling pathway. A Cell viability of cells treated with different concentrations of TLCA for 24 h (n = 10). B & F The mRNA expression of MYH1A and MYH7B (n = 6). C & G Intracellular enzymatic activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), ATP content and mitochondrial DNA (mtDNA) content (n = 6). D & H The mRNA expression of HK1, GPI, PGAM1, PGK1, PYGL, PGC-1α, NRF1 and TFAM (n = 6). E The protein expression of PGC-1α, p38 MAPK and phosphorylation p38 MAPK (n = 3). I The protein expression of PGC-1α (n = 3). Data were shown as mean ± SD; *Represents significant difference when compared with 0 μM TLCA or CON group, and # represents significant difference when compared with TLCA group. *& # P < 0.05, ⁎⁎ & ## P < 0.01, ⁎⁎⁎ & ### P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Expressing, Phospho-proteomics

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Comparative analysis of growth performance and pectoral muscle development of postnatal broiler chickens (n = 6). A The sampling time of AA chickens at day 1 after hatching (D1), D7, D14, D21, D28, D42, D56 and TY chickens at D1, D7, D14, D21, D28, D42, D56, D77, D105. B The changes in body weight of AA and TY chickens. C The changes in pectoral muscle weight of AA and TY chickens. D Immunofluorescence staining of myofibers with MYH1A and MYH7B of AA and TY chickens. Scale bars: 20 μm. E Statistical analysis of immunofluorescence staining density values for pectoral muscle fibers in AA and TY chickens. Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Sampling, Immunofluorescence, Staining

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: Intestinal microbiota transplantation (IMT) affects the growth and pectoral muscle development of chickens. A IMT experimental design. B The variation of body weight of AA and TY chickens during D1 to D28 (n = 6). C Pectoral muscle weight of AA and TY chickens at D21 and D28 (n = 12). d -E Morphology of the myofibers stained by hematoxylin and eosin and the statistics of muscle fiber diameter (MDia) and muscle fiber density (MDen) at D21( D ) and D28 ( E ). F-G The mRNA expression of MYH1A and MYH7B in the pectoral muscle at D21 ( F ) and D28 ( G ) (n = 6). Data were shown as mean ± SD; * P < 0.05, ⁎⁎ P < 0.01, ⁎⁎⁎ P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Transplantation Assay, Staining, Expressing

Journal: Poultry Science

Article Title: Gut microbiota-derived taurolithocholic acid modulates myofiber-type switching via p38 MAPK/PGC-1α signaling underlying breed differences between Arbor Acres and Taoyuan chickens

doi: 10.1016/j.psj.2026.106914

Figure Lengend Snippet: TLCA regulates mitochondrial biogenesis and altered myofiber type composition through the p38 MAPK/PGC-1α signaling pathway. A Cell viability of cells treated with different concentrations of TLCA for 24 h (n = 10). B & F The mRNA expression of MYH1A and MYH7B (n = 6). C & G Intracellular enzymatic activities of succinate dehydrogenase (SDH) and lactate dehydrogenase (LDH), ATP content and mitochondrial DNA (mtDNA) content (n = 6). D & H The mRNA expression of HK1, GPI, PGAM1, PGK1, PYGL, PGC-1α, NRF1 and TFAM (n = 6). E The protein expression of PGC-1α, p38 MAPK and phosphorylation p38 MAPK (n = 3). I The protein expression of PGC-1α (n = 3). Data were shown as mean ± SD; *Represents significant difference when compared with 0 μM TLCA or CON group, and # represents significant difference when compared with TLCA group. *& # P < 0.05, ⁎⁎ & ## P < 0.01, ⁎⁎⁎ & ### P < 0.001.

Article Snippet: Following blocking with 1% BSA, the sections were incubated with the primary antibody MYH1A (1:100, F59, DSHB, USA) and the AF647-labeled goat anti-mouse secondary antibody (1:500, A0473, Beyotime), with the primary antibody MYH7B (1:100, S58, DSHB) and the FITC-labeled goat anti-mouse secondary antibody (1:500, A0568, Beyotime).

Techniques: Expressing, Phospho-proteomics

Journal: PLOS One

Article Title: Alaska pollock protein as a functional dietary source for promoting skeletal muscle hypertrophy and lipid metabolic remodeling

doi: 10.1371/journal.pone.0348366

Figure Lengend Snippet: Immunofluorescence staining of the superficial region of the gastrocnemius muscle at (a) 90 and (b) 210 min post-feeding. Black indicates MyHC IIx–positive fibers, and green indicates MyHC IIb–positive fibers. The cross-sectional area of each fiber was calculated, and data are presented as the mean ± SEM. Statistical comparisons were performed using Student’s t -test (90 min: N = 3; 210 min: N = 4). *; p < 0.05, **; p < 0.01. Scale bar = 100 μm.

Article Snippet: Sections were fixed with acetone and incubated overnight at 4°C with primary antibodies against MyHC IIa (SC-71) and MyHC IIb (BF-F3), obtained from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA, USA).

Techniques: Immunofluorescence, Staining